Crystalline form of n-[5-(3,5-difluoro-benzyl)-1h-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide

ABSTRACT

The present invention relates to a new crystalline form of N-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide, process for its preparation, its utility in treating diseases caused by deregulated protein kinase activity and pharmaceutical compositions containing it.

CROSS REFERENCE TO RELATED APPLICATIONS

This application is a continuation application of U.S. patentapplication Ser. No. 16/301,826, filed on Nov. 15, 2018, which is anational stage entry of PCT/EP2017/061919, filed on May 18, 2017, whichclaims the benefit of U.S. Provisional Application No. 62/340,797 filedon May 24, 2016, the entire contents of which are incorporated herein byreference.

FIELD OF THE INVENTION

The present invention relates to a new crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,process for its preparation, its utility in treating diseases caused byderegulated protein kinase activity and pharmaceutical compositionscontaining it.

BACKGROUND OF THE INVENTION

The malfunctioning of protein kinases (PKs) is the hallmark of numerousdiseases. A large share of the oncogenes and proto-oncogenes involved inhuman cancers encode for PKs. The enhanced activities of PKs are alsoimplicated in many non-malignant diseases, such as benign prostatehyperplasia, familial adenomatosis, polyposis, neuro-fibromatosis,psoriasis, vascular smooth cell proliferation associated withatherosclerosis, pulmonary fibrosis, arthritis, glomerulonephritis andpost-surgical stenosis and restenosis.

PKs are also implicated in inflammatory conditions and in themultiplication of viruses and parasites. PKs may also play a major rolein the pathogenesis and development of neurodegenerative disorders.

For a general reference to PKs malfunctioning or deregulation see, forinstance, Current Opinion in Chemical Biology 1999, 3, 459-465; NatureRev. Drug Discov. 2002; and Carcinogenesis 2008, 29, 1087-1091.

A subset of PK is a group of membrane receptors with intrinsicprotein-tyrosine kinase activity (RPTK). Upon binding of growth factors,RPTKs become activated and phosphorylate themselves and a series ofsubstrates in the cytoplasm. Through this mechanism, they can transduceintracellular signallings for proliferation, differentiation or otherbiological changes. Structural abnormalities, over-expression andactivation of RPTKs are frequently observed in human tumors, suggestingthat constitutive ignition of the signal transduction leading to cellproliferation can result in malignant transformation. Anaplasticlymphoma kinase (ALK) is a tyrosine kinase receptor belonging to theinsulin receptor subfamily of RPTKs: the ALK gene is located oncromosome 2 and is expressed mainly in neuronal cells, especially duringdevelopment. The ALK gene is involved in a balanced chromosomaltranslocation with the Nucleophosmin (NPM) gene on cromosome 5 in alarge subset of Anaplastic Large Cell Lymphomas (ALCL). In the ALK+ALCL,as a result of the translocation, the NPM ubiquitous promoter drives anectopic expression of the fusion protein in which the NPM moietydimerizes and the ALK kinase domain undergoes auto-phosphorylation andbecomes constitutively active.

Many data from the literature have demonstrated that the NPM-ALK fusionprotein has a strong oncogenic potential and its ectopic expression isresponsible for cellular transformation. Moreover, the constitutiveexpression of human NPM-ALK in mouse T-cell lymphocytes is sufficientfor the development of lymphoid neoplasia in transgenic animals with ashort period of latency.

ALCL is a defined disease characterized by the surface expression of theCD30 antigen (Ki-1), and accounts for 2% of adult and 13% of pediatricnon-Hodgkin's lymphomas, affecting predominantly young male patients.ALK+ ALCL accounts for 70% of all ALCLs and is an aggressive diseasewith systemic signs, and frequent extranodal involvement (bone marrow,skin, bone, soft tissues).

About 15-20% of ALK-expressing ALCLs were found to bear a differentchromosomal translocation, involving the cytoplasmic portion of ALK,with different N-terminal moieties, all resulting in constitutiveactivation of the ALK kinase domain.

Moreover, cell lines established from solid tumors of etodermal originlike melanomas, breast carcinomas, as well as neuroblastomas,glioblastomas, Ewings sarcomas, retinoblastomas, were found to expressthe ALK receptor.

ROS1 belongs to the insulin-receptor superfamily. Like other tyrosinekinase receptor molecules, it plays a role in relaying growth signalsfrom the environment outside the cell into the cell's nucleus. It is 1of 2 orphan receptor tyrosine kinase family members with no knownbinding ligand. Genetic changes in ROS1, such as gene rearrangements,mutations, or copy number increases, create oncogenes, which can lead tocancer (Stumpfova and Janne, 2012). ROS1 was discovered in NSCLCpatients in the form of a fusion protein (6 different partners for ROS1)and is found in approximately 2% of patients with NSCLC (Bergethon etal., 2012; Davies et al, 2012). Two other ROS1 gene rearrangements havebeen detected in a variety of other canoers, including glioblastomamultiforme, cholangiocarcinoma, ovarian cancer, gastric adenocarcinoma,colorectal cancer, inflammatory myofibroblastic tumor, angiosarcoma, andepitheloid hemangioendothelioma (Lee et al., 2013; Davies and Doebele,2013; Davies, et al., 2012; Shaw et al., 2013).

ROS1 gene rearrangements create fusion proteins with constitutivelyactive kinase domains that activate downstream signaling pathwaysleading to onoogenic properties in cells, including uncontrolledproliferation and resistance to cell death with prolonged tumor cellsurvival. These pathways include Ras-ERK for cellular proliferation andthe JAK-STAT and PI3K/AKT pathways, which regulate cell survival(anti-apoptosis) and proliferation. ROS1 fusion proteins may alsoactivate the mTOR pathway, which is critical for the regulation ofprotein translation. Cancers that have these pathways activated tend tobe more aggressive, with invasion and metastasis leading to poorsurvival of the patients (Davies and Doebele, 2013).

Trk's are the high affinity receptor tyrosine kinases activated by agroup of soluble growth factors called neurotrophins (NT). The Trkreceptor family has three members-TrkA, TrkB and TrkC. Among theneurotrophins are (i) nerve growth factor (NGF) which activates TrkA,(ii) brain-derived neurotrophic factor (BDNF) and NT-4/5 which activateTrkB and (iii) NT3 which activates TrkC. Trk's are widely expressed inneuronal tissue and are implicated in the maintenance, signaling andsurvival of neuronal cells (Patapoutian, A. et al., Current Opinion inNeurobiology, 2001, 11, 272-280). NTRK1 encodes the TrkA receptortyrosine kinase. TrkA activates the PI3K/AKT, PKC and ERK1/2 pathwayswhich promote cell growth and survival.

Recent literature has also shown that overexpression, activation,amplification and/or mutation of Trk's are associated with many cancersincluding neuroblastoma (Brodeur, G. M., Nat. Rev. Cancer 2003, 3,203-216), ovarian cancer (Davidson. B., et al., Clin. Cancer Res. 2003,9, 2248-2259), breast cancer (Kruettgen et al, Brain Pathology 2006, 16:304-310), prostate cancer (Dionne et al, Clin. Cancer Res. 1998, 4(8):1887-1898), pancreatic cancer (Dang et al, Journal of Gastroenterologyand Hepatology 2006, 21(5): 850-858), multiple myeloma (Hu et al, CancerGenetics and Cytogenetics 2007, 178: 1-10), astrocytoma andmedulloblastoma (Kruettgen et al, Brain Pathology 2006, 16: 304-310)glioma (Hansen et al, Journal of Neurochemistry 2007, 103: 259-275),melanoma (Truzzi et al, Journal of Investigative Dermatology 2008,128(8): 2031-2040, thyroid carcinoma (Brzezianska et al,Neuroendocrinology Letters 2007, 28(3), 221-229), lung adenocarcinoma(Perez-Pinera et al, Molecular and Cellular Biochemistry 2007, 295(1&2),19-26), large cell neuroendocrine tumors (Marchetti et al, HumanMutation 2008, 29(5), 609-616), and colorectal cancer (Bardelli, A.,Science 2003, 300, 949). In preclinical models of cancer, Trk inhibitorsare efficacious in both inhibiting tumor growth and stopping tumormetastasis. In particular, non-selective small molecule inhibitors ofTrk A, B and C and Trk/Fc chimeras were efficacious in both inhibitingtumor growth and stopping tumor metastasis (Nakagawara, A. (2001) CancerLetters 169:107-114; Meyer, J. et al. (2007) Leukemia, 1-10; Pierotti,M. A and Greco A., (2006) Cancer Letters 232:90-98; Eric Adriaenssens,E. et al. Cancer Res (2008) 68:(2) 346-351) (Truzzi et al, Journal ofInvestigative Dermatology 2008, 128(8): 2031-2040. Therefore, aninhibitor of the Trk family of kinases is expected to have utility inthe treatment of cancer.

In conclusion, interfering with ALK or ROS1 signaling likely representsa specific and effective way to block tumor cell proliferation in ALCLand possibly other indications. The insulin-like growth factor 1receptor (IGF-1R, IGF1R) is also a member of the insulin receptorsubfamily of RTKs. In addition, interfering with TrkA, TrkB and/or TrkCsignaling, or a combination thereof, represents a specific and effectiveway to block tumor cell proliferation in various cancers, including, butnot limited to, non-small cell lung cancer, papillary thyroid cancer,neuroblastoma, pancreatic cancer and colorectal cancer.

SUMMARY OF THE INVENTION

The international patent application WO2009/013126 (in the name ofNerviano Medical Sciences SrI) describes and claims the free-base formofN-[5-(3,5-Difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl-2-(tetrahydro-pyran-4-ylamino)-benzamide,which has the following formula (I)

has been assigned Chemical Abstracts Registry No. 1108743-60-7 and hasbeen assigned the international non-proprietary name (INN) entrectinib.

The compound of formula (i) is active as a kinase inhibitor, moreparticularly an inhibitor of ALK, ROS1, TrkA, TrkB, and Trk C kinases,and it is thus useful in the treatment of a variety of cancers and cellproliferative disorders as disclosed in international patent applicationWO2015124697 (in the name of Ignyta Inc. and Nerviano Medical SciencesSrI) and as demonstrated by clinical trials in human patients havingcancer. Entrectinib has been tested clinically in cancer patients withvarious types of cancerous, solid tumors, the cells of which contain oneor more genetic alterations in at least one target gene in the cancerpatient, wherein the at least one target gene is selected from ALK,ROS1, SORT1, NTRK1, NTRK2, and NTRK3. Aug. 15, 2015, the findings ofsuch clinical trials showed that a total of 92 patients with a range ofsolid tumors had been dosed across both clinical trials, with 9 patientstreated at or above the recommended Phase II dose beyond six months and1 patient beyond one year. Across the Phase I studies, the most frequent(>10% incidence) treatment-related adverse events were fatigue,dysgeusia, paresthesia, nausea, and myalgia. Seven of these were Grade 3in severity, consisting of fatigue (4 patients), cognitive impairment (2patients), and diarrhea (1 patient). No Grade 4 or Grade 5treatment-related adverse events were observed. Across the Phase Istudies, there were three treatment-related serious adverse events:Grade 3 cognitive impairment and Grade 3 myocarditis, both of whichoccurred at doses above the recommended Phase II dose, and Grade 2fatigue. All events were reversible and resolved upon dose modification.Pharmacokinetic measurements showed dose-proportional increases acrossthe daily dosing regimens evaluated, with a half-life compatible withonce-daily dosing. The recommended Phase II dose was determined to be600 mg, taken orally once per day. A total of 18 patients across thePhase I studies met our expected Phase 2 eligibility criteria, whichinclude the presence of NTRK1, NTRK2, NTRK3, ROS1 or ALK geneticalterations which are fusions, as opposed to other types of molecularalterations; the patient not having been previously treated with an ALKinhibitor or a ROS1 inhibitor and treatment at or above the recommendedPhase II dose. In the Phase I clinical trials, the response rate,defined as complete and partial responses by Response EvaluationCriteria In Solid Tumors, or RECIST criteria, in the 18 patients who metthe expected Phase 2 eligibility criteria across both studies was 72%,based on 13 responses out of 18 treated patients, as assessed by theclinical sites. Nine of these responders remained on study treatmentwith durable responses of up to 21 treatment cycles. An additional 3patients remained on study with stable disease. The responses included(a) 3 responses out of 4 patients with NTRK1, NTRK2 or NTRK3 generearrangements, including patients with non-small cell lung cancer, orNSCLC, colorectal cancer and salivary gland cancer, with one of theresponding patients remaining on treatment at 6 months; a fourth patientwith an astrocytoma remains on treatment after two months with stabledisease, (b) 6 responses, including one complete response, out of 8patients with ROS1 gene rearrangements, all of which were in NSCLC. Allof the patients who responded remain on treatment, the longest at 21months, and (c) 4 responses out of 6 patients with ALK generearrangements, including two NSCLC patients and two patients with othersolid tumors; 2 of the 4 responders subsequently progressed.

Two preparations of this compound are described respectively in example2 (step i′) and example 7 of the above cited application WO2009/013126.The process described in example 2 comprises, essentially, adding5-(3,5-difluoro-benzyl)-1H-indazol-3-ylamine to4-(4-methyl-piperazin-1-yl)-2-[(tetrahydro-pyran-4-yl)-(2,2,2-trifluoro-acetyl)-amino]-benzoicacyl chloride and then deprotecting with an organic base at hightemperature to give the desired amide of formula (I), after purificationby column chromatography and crystallization.

The other process described in example 7 of the above cited patentapplication is a conversion which comprises, essentially, reacting2-amino-N-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-benzamidewith tetrahydro-pyran-4-one in presence of trifluoroacetic acid andtetramethylammonium triacetoxyborohydride to give the desired amide offormula (I), after purification by column chromatography.

However, the solid form of these preparations was not disclosed in theWO20091013126 application. The products obtained by following theexperimental procedures in example 2 (step i′) and example 7 describedin application WO2009/013126 were disclosed in application WO2013/174876(in the name of Nerviano Medical Sciences SrI). In particular, examplethe procedure disclosed in example 2 (step i′) was found to yield acrystalline solvate of the compound of formula (I) containing ethylacetate and n-hexane (hereinafter referred to as “Form 3”). Followingthe experimental procedures in example 7 of the WO2009/013126application was found to yield an amorphous form of the compound offormula (I).

Two novel solid forms of the compound of formula (I) have been disclosedin application WO2013/174876, together with a process for theirpreparation, which provides the product in an industrially advantageousand highly reproducible manner with high purity and characteristicssuitable for administration to humans and at a reduced cost. This newprocess was characterized by reaction of an acyl chloride with anindazol-3-ylamine followed by deprotection under mild basic conditionsand treatment of the final amorphous compound with a mixture of ethanoland water in presence of seeds, to give the desired compound of formula(I) in crystalline a form designated “form 1” or with a mixture ofethanol and water, to give the desired compound of formula (I) in acrystalline form designated “form 2.” The crystalline, solvate form ofthe compound of formula (I), which is a solvate of ethyl acetate andhexane found to have resulted from following the experimental procedurein application WO2009/013126 was also disclosed in applicationWO2013/174876 and was designated as “form 3” therein.

However there is still a need in therapy to understand the polymorphicbehaviour of the potential pharmaceutical compound defined above and tostandardize the methods for obtaining its different solid forms. Infact, the identification and characterization of the polymorphic formstogether with the definitions of the experimental conditions forobtaining them is very important for a compound endowed withpharmacological activity like the compound of formula (I) defined above,because the polymorphism of a compound that could be used as activeingredient in a pharmaceutical composition can influence many aspects ofthe final drug, such as the pharmacokinetic and bioavailability, itstoxicological profile, solubility and other phisico-chemical propertiesof the bulk powder.

In this respect, it has been now unexpectedly and surprisingly foundthat the above compound of formula (I) may also exist in a crystalline,unsolvated form not previously disclosed. This novel crystalline form ofcompound (I) may be referred to herein, unless otherwise specified, as“crystalline Form 4” or “Form 4” of the compound of formula (I).

It has been surprisingly found that Form 4 of the compound of formula(I) is exhibits greater thermodynamic stability at temperature of about40° C. or higher vis-à-vis the three known crystalline forms disclosedin WO20131174876, one of which is a solvate with ethyl acetate andhexane. Form 4 of the compound of formula (I) may also offer advantagesin the preparation of dosage forms or medicaments useful for thetreatment of cancer patients with various types of cancerous, solidtumors, the cells of which contain one or more genetic alterations in atleast one target gene, wherein the at least one target gene is selectedfrom ALK, ROS1, SORT1, NTRK1, NTRK2, and NTRK3. Form 4 of the compoundof formula (I) may also offer advantages in the methods of treatment ofpatients having cancer, comprising administering to the patient atherapeutically effective amount of Form 4 of the compound of formula(I) to the patient.

Preparation of dosage forms or medicaments useful for the treatment ofcancer patients with various types of cancerous, solid tumors, the cellsof which contain one or more genetic alterations in at least one targetgene, wherein the at least one target gene is selected from ALK, ROS1,SORT1, NTRK1, NTRK2, and NTRK3.

The new crystalline Form 4 has been characterized through the x-raypowder diffraction (XRPD) and differential scanning calorimetry (DSC)techniques.

DESCRIPTION OF THE DRAWINGS

The invention is also illustrated by reference to the accompanyingdrawings described below.

FIG. 1 shows the x-ray powder diffraction pattern (XRPD) of thecrystalline Form 4; 2-Theta angles (deg) are reported on the x axiswhile intensity (CPS) is reported on the y axis.

FIG. 2 shows the differential scanning calorimetry (DSC) thermogram ofthe crystalline Form 4. The thermogram reports temperature (° C.) andtime (min) on the x axis, while heat flow (mW) is reported on the yaxis.

DETAILED DESCRIPTION

As used herein, the term“N-[5-(3,5-Difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide”means the compound having the chemical structure:

Which has been assigned Chemical Abstracts Registry No. 1108743-60-7 andhas been assigned the international non-proprietary name (INN)entrectinib.

The use of the term “about” includes and describes the value orparameter per se. For instance, “about x” includes and describes “x” perse. In some embodiments, the term “about” when used in association witha measurement, or used to define a value, a unit, a constant, or a rangeof values, refers to a variation ±10%, unless indicated otherwise. Forexample, “about 40° C.” in some embodiments includes 36° C. to 44° C.

The use of the term “adding” herein does not limit the order, method orhow the materials being added are combined. For instance, “adding A toB” may also describe “adding B to A”.

As used herein, the terms “administration” and “administering” mean thedelivery of a bioactive composition or formulation to a subject by anadministration route including, but not limited to, oral, intravenous,intra-arterial, intramuscular, intraperitoneal, subcutaneous,intramuscular, topically, or combinations thereof. In some embodiments,the administration to a subject is oral.

As used herein, the term “ALK” means anaplastic lymphoma kinase receptoror CD246 (cluster of differentiation 246), which is an enzyme that inhumans is encoded by the ALK gene and also has the UniProt identifiedALK_HUMAN.

As used herein, the terms “cancer” or “tumor” may be usedinterchangeably. These terms mean the presence of cells possessingcharacteristics typical of cancer-causing cells, such as uncontrolledproliferation, immortality, metastatic potential, rapid growth andproliferation rate, and certain characteristic morphological features.Cancer cells are often in the form of a tumor, but such cells can existalone within an animal, or can be a non-tumorigenic cancer cell, such asa leukemia cell. These terms include a solid tumor, a soft tissue tumor,or a metastatic lesion. As used herein, the term “cancer” includespremalignant, as well as malignant cancers. In certain embodiments, thecancer is a solid tumor, a soft tissue tumor, or a metastatic lesion.The terms also refer to solid tumors named for the type of cells thatform them, cancer of blood, bone marrow, or the lymphatic system.Examples of solid tumors include, but are not limited to, sarcomas andcarcinomas. Examples of cancers of the blood include, but are notlimited to, leukemias, lymphomas and myeloma. The terms include, but arenot limited to, a primary cancer that originates at a specific site inthe body, a metastatic cancer that has spread from the place in which itstarted to other parts of the body, a recurrence from the originalprimary cancer after remission, and a second primary cancer that is anew primary cancer in a person with a history of previous cancer ofdifferent type from latter one. As used herein “cancer” refers to anymalignant and/or invasive growth or tumor caused by abnormal cellgrowth.

As used herein, the term “crystalline” means a solid phase in which amaterial has a regular ordered internal structure at the molecular leveland gives a distinctive x-ray diffraction pattern with defined peaks.

The term “other therapeutic agents” as used herein means, but is notlimited to, antihormonal agents such as antiestrogens, antiandrogens andaromatase inhibitors, topoisomerase I inhibitors, topoisomerase IIinhibitors, agents that target microtubules, platin-based agents,alkylating agents, DNA damaging or intercalating agents, antineoplasticantimetabolites, other kinase inhibitors, other anti-angiogenic agents,inhibitors of kinesins, therapeutic monoclonal antibodies, inhibitors ofmTOR, histone deacetylase inhibitors, farnesyl transferase inhibitors,and inhibitors of hypoxic response.

The terms “patient” and/or “subject” as used herein mean animal, such asmammal (including human), that has been or will be the object oftreatment, observation or experiment. In some embodiments, the subjector patient is a human.

The term “pharmaceutical acceptable excipient” means diluents,disintegrants, precipitation inhibitors, surfactants, glidants,lubricants and other excipients and vehicles with which a compound isadministered to a subject or patient.

As used herein, “ROS1” means the ROS1 receptor tyrosine-protein kinasehaving the UniProt designation ROS1_HUMAN and encoded by the ROS1 gene.

The term “therapeutically effective amount” as used herein means theamount of a compound or pharmaceutical composition sufficient to effecttreatment when administered to a subject, to provide a therapeuticbenefit such as amelioration of symptoms or slowing of diseaseprogression. For example, a therapeutically effective amount may be anamount sufficient to decrease a symptom of a disease or conditionresponsive to inhibition of ALK, TrkA, TrkB, TrkC, and ROS1 activity ora combination thereof.

The term “tropomyosin receptor kinase,” as used herein, means the familyof tropomyosin receptor kinases (Trks) that are activated by peptidehormones of the neurotrophin family and include, but are not limited to,TrkA, TrkB, and TrkC. As used herein, the term “TrkA” means wild-typetropomyosin receptor kinase A having the UniProt identifier NTRK1_HUMANand encoded by the NTRK1 gene. As used herein, the term “TrkB” meanswild-type tropomyosin receptor kinase B having the UniProt identifierNTRK2_HUMAN and encoded by the NTRK2 gene. As used herein, the term“TrkC” means wild-type tropomyosin receptor kinase C having the UniProtidentifier NTRK3_HUMAN and encoded by the NTRK3 gene. TrkA, TrkB andTrkC are also referred to by those having ordinary skill in the art asTrk1, Trk2 and Trk3, respectively. A reference to TrkA is a reference toTrk1. A reference to TrkB is a reference to Trk2. A reference to TrkC isa reference to Trk3.

In one embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising a peak at a 2-theta value of about 8.6degrees. In another embodiment is provided such a crystalline form,wherein said crystalline form is unsolvated.

In another embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising a peak at a 2-theta value of about8.6±0.5 degrees. In another embodiment is provided such a crystallineform, wherein said crystalline form is unsolvated.

In another embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising a peak at a 2-theta value of about8.6±0.2 degrees. In another embodiment is provided such a crystallineform, wherein said crystalline form is unsolvated.

In one embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising a peak at a 2-theta value of about 15.6degrees. In another embodiment is provided such a crystalline form,wherein said crystalline form is unsolvated.

In another embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising a peak at a 2-theta value of about15.6±0.5 degrees. In another embodiment is provided such a crystallineform, wherein said crystalline form is unsolvated.

In another embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising a peak at a 2-theta value of about15.6±0.2 degrees. In another embodiment is provided such a crystallineform, wherein said crystalline form is unsolvated.

In one embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising a peak at a 2-theta value of about 17.9degrees. In another embodiment is provided such a crystalline form,wherein said crystalline form is unsolvated.

In another embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising a peak at a 2-theta value of about17.9±0.5 degrees. In another embodiment is provided such a crystallineform, wherein said crystalline form is unsolvated.

In another embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising a peak at a 2-theta value of about17.9±0.2 degrees. In another embodiment is provided such a crystallineform, wherein said crystalline form is unsolvated.

In another embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising peaks at a 2-theta value of about 15.6and 17.9 degrees. In another embodiment is provided such a crystallineform, wherein said crystalline form is unsolvated.

In another embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising peaks at a 2-theta value of about 15.6and 17.9±0.5 degrees. In another embodiment is provided such acrystalline form, wherein said crystalline form is unsolvated.

In another embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising peaks at a 2-theta value of about 15.6and 17.9±0.2 degrees. In another embodiment is provided such acrystalline form, wherein said crystalline form is unsolvated.

In another embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising peaks at a 2-theta value of about 15.6,17.9 and 39.0 degrees. In another embodiment is provided such acrystalline form, wherein said crystalline form is unsolvated.

In another embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising peaks at a 2-theta value of about 15.6,17.9 and 39.0±0.5 degrees. In another embodiment is provided such acrystalline form, wherein said crystalline form is unsolvated.

In another embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising peaks at a 2-theta value of about 15.6,17.9 and 39.0±0.2 degrees. In another embodiment is provided such acrystalline form, wherein said crystalline form is unsolvated.

In another embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising peaks at a 2-theta value of about 8.6,10.3, 11.0, 11.9, 14.3, 14.6, 15.1, 15.3, 15.6, 16.1, 17.1, 17.9, 19.0,19.2, 19.6, 19.7, 20.1, 20.7, 21.3, 22.1, 22.7, 24.3, 24.6, 25.3, 25.5,25.9, 26.7, 26.9, 27.3, 27.7, 28.1, 28.6, 29.0, 29.5, 29.9, 30.5, 31.0,31.6, 32.2, 33.3, 34.0, 35.4, 36.4, 36.8 and 39.0 degrees. In anotherembodiment is provided such a crystalline form, wherein said crystallineform is unsolvated.

In another embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising peaks at a 2-theta value of about 8.6,10.3, 11.0, 11.9, 14.3, 14.6, 15.1, 15.3, 15.6, 16.1, 17.1, 17.9, 19.0,19.2, 19.6, 19.7, 20.1, 20.7, 21.3, 22.1, 22.7, 24.3, 24.6, 25.3, 25.5,25.9, 26.7, 26.9, 27.3, 27.7, 28.1, 28.6, 29.0, 29.5, 29.9, 30.5, 31.0,31.6, 32.2, 33.3, 34.0, 35.4, 36.4, 36.8 and 39.0±0.5 degrees. Inanother embodiment is provided such a crystalline form, wherein saidcrystalline form is unsolvated.

In another embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising peaks at a 2-theta value of about 8.6,10.3, 11.0, 11.9, 14.3, 14.6, 15.1, 15.3, 15.6, 16.1, 17.1, 17.9, 19.0,19.2, 19.6, 19.7, 20.1, 20.7, 21.3, 22.1, 22.7, 24.3, 24.6, 25.3, 25.5,25.9, 26.7, 26.9, 27.3, 27.7, 28.1, 28.6, 29.0, 29.5, 29.9, 30.5, 31.0,31.6, 32.2, 33.3, 34.0, 35.4, 36.4, 36.8 and 39.0 degrees±0.2 degrees.In another embodiment is provided such a crystalline form, wherein saidcrystalline form is unsolvated.

In another embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by exhibiting a peak in adifferential scanning calorimetry scan of from about 200° C. to about205° C.

In another embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by exhibiting a peak in adifferential scanning calorimetry scan of from about 200° C. to about205° C., and an x-ray powder diffraction pattern comprising a peak at a2-theta value of about 15.6 degrees. In another embodiment is providedsuch a crystalline form, wherein said crystalline form is unsolvated.

In another embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by exhibiting a peak in adifferential scanning calorimetry scan of from about 200° C. to about205° C., and an x-ray powder diffraction pattern comprising a peak at a2-theta value of about 15.6±0.5 degrees. In another embodiment isprovided such a crystalline form, wherein said crystalline form isunsolvated.

In another embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by exhibiting a peak in adifferential scanning calorimetry scan of from about 200° C. to about205° C., and an x-ray powder diffraction pattern comprising a peak at a2-theta value of about 15.6±0.2 degrees. In another embodiment isprovided such a crystalline form, wherein said crystalline form isunsolvated.

In another embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by exhibiting a peak in adifferential scanning calorimetry scan of from about 200° C. to about205° C., and an x-ray powder diffraction pattern comprising a peak at a2-theta value of about 15.6 and 17.9 degrees. In another embodiment isprovided such a crystalline form, wherein said crystalline form isunsolvated.

In another embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methy-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by exhibiting a peak in adifferential scanning calorimetry scan of from about 200° C. to about205° C., and an x-ray powder diffraction pattern comprising a peak at a2-theta value of about 15.6 and 17.9±0.5 degrees. In another embodimentis provided such a crystalline form, wherein said crystalline form isunsolvated.

In another embodiment is provided a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by exhibiting a peak in adifferential scanning calorimetry scan of from about 200° C. to about205° C., and an x-ray powder diffraction pattern comprising a peak at a2-theta value of about 15.6 and 17.9±0.2 degrees. In another embodimentis provided such a crystalline form, wherein said crystalline form isunsolvated.

In another embodiment is provided a pharmaceutical compositioncomprising a therapeutically effective amount of a crystalline form ofN-[5-(3,5-Difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising a peak at a 2-theta value of about 15.6degrees, and one or more pharmaceutically acceptable excipients.

In another embodiment is provided a pharmaceutical compositioncomprising a therapeutically effective amount of a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising a peak at a 2-theta value of about15.6±0.5 degrees, and one or more pharmaceutically acceptableexcipients.

In another embodiment is provided a pharmaceutical compositioncomprising a therapeutically effective amount of a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising a peak at a 2-theta value of about15.6±0.2 degrees, and one or more pharmaceutically acceptableexcipients.

In another embodiment is provided a pharmaceutical compositioncomprising a therapeutically effective amount of a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising peaks at a 2-theta value of about 15.6and 17.9 degrees.

In another embodiment is provided a pharmaceutical compositioncomprising a therapeutically effective amount of a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising peaks at a 2-theta value of about 15.6and 17.9±0.5 degrees.

In another embodiment is provided a pharmaceutical compositioncomprising a therapeutically effective amount of a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising peaks at a 2-theta value of about 15.6and 17.9±0.2 degrees.

In another embodiment is provided a pharmaceutical compositioncomprising a therapeutically effective amount of a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by exhibiting a peak in adifferential scanning calorimetry scan of from about 200° C. to about205° C.

In another embodiment is provided a pharmaceutical compositioncomprising a therapeutically effective amount of a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by exhibiting a peak in adifferential scanning calorimetry scan of from about 200° C. to about205° C., and an x-ray powder diffraction pattern comprising a peak at a2-theta value of about 15.6 degrees.

In another embodiment is provided a pharmaceutical compositioncomprising a therapeutically effective amount of a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by exhibiting a peak in adifferential scanning calorimetry scan of from about 200° C. to about205° C., and an x-ray powder diffraction pattern comprising a peak at a2-theta value of about 15.6±0.5 degrees.

In another embodiment is provided a pharmaceutical compositioncomprising a therapeutically effective amount of a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by exhibiting a peak in adifferential scanning calorimetry scan of from about 200° C. to about205° C., and an x-ray powder diffraction pattern comprising a peak at a2-theta value of about 15.6±0.2 degrees.

In another embodiment is provided a pharmaceutical compositioncomprising a therapeutically effective amount of a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by exhibiting a peak in adifferential scanning calorimetry scan of from about 200° C. to about205° C., and an x-ray powder diffraction pattern comprising a peak at a2-theta value of about 15.6 and 17.9 degrees. In another embodiment isprovided such a crystalline form, wherein said crystalline form isunsolvated.

In another embodiment is provided a pharmaceutical compositioncomprising a therapeutically effective amount of a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by exhibiting a peak in adifferential scanning calorimetry scan of from about 200° C. to about205° C., and an x-ray powder diffraction pattern comprising a peak at a2-theta value of about 15.6 and 17.9±0.5 degrees.

In another embodiment is provided a pharmaceutical compositioncomprising a therapeutically effective amount of a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by exhibiting a peak in adifferential scanning calorimetry scan of from about 200° C. to about205° C., and an x-ray powder diffraction pattern comprising a peak at a2-theta value of about 15.6 and 17.9±0.2 degrees.

In another embodiment is provided method of treating a patient havingcancer, comprising administering to said patient a therapeuticallyeffective amount of a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising a peak at a 2-theta value of about 15.6degrees.

In another embodiment is provided method of treating a patient havingcancer, comprising administering to said patient a therapeuticallyeffective amount of a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising a peak at a 2-theta value of about15.6±0.5 degrees.

In another embodiment is provided method of treating a patient havingcancer, comprising administering to said patient a therapeuticallyeffective amount of a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising a peak at a 2-theta value of about15.6±0.2 degrees.

In another embodiment is provided method of treating a patient havingcancer, comprising administering to said patient a therapeuticallyeffective amount of a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising peaks at a 2-theta value of about 15.6and 17.9 degrees.

In another embodiment is provided method of treating a patient havingcancer, comprising administering to said patient a therapeuticallyeffective amount of a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising peaks at a 2-theta value of about 15.6and 17.9±0.5 degrees.

In another embodiment is provided method of treating a patient havingcancer, comprising administering to said patient a therapeuticallyeffective amount of a crystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising peaks at a 2-theta value of about 15.6and 17.9±0.2 degrees.

In another embodiment is provided method of treating a patient havingcancer, comprising administering to said patient a pharmaceuticalcomposition comprising a therapeutically effective amount of acrystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising a peak at a 2-theta value of about 15.6degrees, and one or more pharmaceutically acceptable excipients.

In another embodiment is provided method of treating a patient havingcancer, comprising administering to said patient a pharmaceuticalcomposition comprising a therapeutically effective amount of acrystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising a peak at a 2-theta value of about15.6±0.5 degrees, and one or more pharmaceutically acceptableexcipients.

In another embodiment is provided method of treating a patient havingcancer, comprising administering to said patient a pharmaceuticalcomposition comprising a therapeutically effective amount of acrystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising a peak at a 2-theta value of about15.6±0.2 degrees, and one or more pharmaceutically acceptableexcipients.

In another embodiment is provided method of treating a patient havingcancer, comprising administering to said patient a pharmaceuticalcomposition comprising a therapeutically effective amount of acrystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising peaks at a 2-theta value of about 15.6and 17.9 degrees.

In another embodiment is provided method of treating a patient havingcancer, comprising administering to said patient a pharmaceuticalcomposition comprising a therapeutically effective amount of acrystalline form ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising peaks at a 2-theta value of about 15.6and 17.9±0.5 degrees.

In another embodiment is provided method of treating a patient havingcancer, comprising administering to said patient a therapeuticallyeffective amount of pharmaceutical composition comprising a crystallineform ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,wherein said crystalline form is characterized by an x-ray powderdiffraction pattern comprising peaks at a 2-theta value of about 15.6and 17.9±0.2 degrees.

In other embodiments are provided any of the methods of treating apatient having cancer described herein wherein said cancer is selectedfrom breast cancer, lung cancer, colorectal cancer, prostate cancer,ovarian cancer, endometrial cancer, gastric cancer, clear cell renalcell carcinoma, invasive ductal carcinoma (breast), uveal melanoma,multiple myeloma, rhabdomyosarcoma, Ewing's sarcoma, Kaposi's sarcoma,pancreatic cancer, medulloblastoma, anaplastic large cell lymphomas(ALCL), neuroblastoma, rhabdomyosarcoma, glioblastoma, inflammatorymyofibroblastic tumor, melanoma, retinoblastoma, non-small cell lungcancer (NSCLC), salivary gland cancer, and mammary analog secretorycarcinoma (MASC).

In other embodiments are provided any of the methods of treating apatient having cancer described herein wherein said cancer is selectedfrom breast cancer, lung cancer, colorectal cancer, prostate cancer,gastric cancer, medulloblastoma, neuroblastoma, glioblastoma, melanoma,non-small cell lung cancer (NSCLC), salivary gland cancer, and mammaryanalog secretory carcinoma (MASC).

In other embodiments are provided any of the methods of treating apatient having cancer described herein wherein said cancer is selectedfrom breast cancer, lung cancer, colorectal cancer, medulloblastoma,neuroblastoma, glioblastoma, melanoma, non-small cell lung cancer(NSCLC), salivary gland cancer, and mammary analog secretory carcinoma(MASC).

In other embodiments are provided any of the methods of treating apatient having cancer described herein wherein said cancer is selectedfrom colorectal cancer, neuroblastoma, glioblastoma, melanoma, non-smallcell lung cancer (NSCLC), salivary gland cancer, and mammary analogsecretory carcinoma (MASC).

In other embodiments are provided any of the methods of treating apatient having cancer described herein wherein said cancer is colorectalcancer.

In other embodiments are provided any of the methods of treating apatient having cancer described herein wherein said cancer isneuroblastoma.

In other embodiments are provided any of the methods of treating apatient having cancer described herein wherein said cancer isglioblastoma.

In other embodiments are provided any of the methods of treating apatient having cancer described herein wherein said cancer is melanoma.

In other embodiments are provided any of the methods of treating apatient having cancer described herein wherein said cancer is non-smallcell lung cancer (NSCLC).

In other embodiments are provided any of the methods of treating apatient having cancer described herein wherein said cancer is salivarygland cancer.

In other embodiments are provided any of the methods of treating apatient having cancer described herein wherein said cancer is mammaryanalog secretory carcinoma (MASC).

In other embodiments are provided any of the methods of treating apatient having cancer described herein, wherein said cancer is locallyadvanced or metastatic.

The present invention also discloses a method of preparation of thecrystalline Form 4 defined above comprising the following steps:

a) adding the acyl chloride of formula (II):

to the indazol-3-ylamine of formula (III):

blocking the addition when the indazol-3-ylamine of formula (III) iscompletely reacted;b) deprotecting under mild basic conditions the resulting compound offormula (IV):

to obtain the compound of formula (I) as defined above;c) suspending in ethanol the obtained compound of formula (I);d) heating the suspension of step c) to a temperature between about 50°C. and about 70° C. and cooling to room temperature; ande) adding water, filtering and drying to give the crystalline form 4 ofcompound of formula (I), as defined above.

Preferably, the suspension obtained in step c) is heated to atemperature of about 60° C.

The present invention also discloses a method of preparation of thecrystalline Form 4 defined above by conversion of Form 2 into Form 4,which is facilitated by adding an amount of crystalline Form 4 seeds toForm 2.

Accordingly, provided herein is a process for preparing the crystallineForm 4 ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,which comprises:

f) heating at reflux temperature (about 78° C.) a slurry of crystallineForm 2 of compound of formula (I) as defined above in ethanol andcooling the mixture to a temperature between about 50° C. and about 65°C.;g) adding an amount of crystalline Form 4 seeds, obtained as defined insteps a) to e), to the mixture of step f), followed by adding water;h) heating the mixture of step g) at reflux temperature for a timeperiod ranging from 30 to 90 minutes, followed by cooling the mixture toroom temperature and stirring for about 12 hours; andi) filtering the mixture of step h) and drying at about 40° C. to obtainthe crystalline Form 4 of the compound of formula (I) as defined above.

Preferably, it is provided a process as defined above wherein thesolution of step f) is cooled at temperature between 55° C. and 60° C.

In another aspect, it is provided a process as defined above, whereinthe mixture of step g) is heated at reflux temperature for about 60minutes.

Preferably, the amount of Form 4 seeds added to crystalline Form 2 isbetween 0.05 and 0.5 weight percent of crystalline Form 2. Morepreferably, the amount of Form 4 seeds added to crystalline Form 2 isabout 0.2 weight percent of crystalline Form 2.

In another aspect, provided is a crystalline Form 4, as defined above,prepared by any of the foregoing methods.

The preparation of the compound of formula (I) in crystalline Form 2 isdescribed in the above cited patent application WO20131174876.

The crystalline Form 4 as defined above, can be subsequently formulatedwith a pharmaceutically acceptable carrier or diluent to provide apharmaceutical composition.

According to steps a) and b) the reaction conditions are the same asdescribed in WO20131174876 on page 4, lines 15-28.

According to step c) the amorphous compound of formula (I), obtained instep b), is suspended in ethanol.

According to step d), the suspension thus obtained is heated to atemperature between about 50° C. and about 70° C., preferably to about60° C., until a solution is obtained and then it is cooled to roomtemperature.

According to step e), to the suspension obtained from step d) is addedwater and then the resultant precipitate is filtered and dried undervacuum to get crystalline Form 4 of compound of formula (I).

According to step f), a slurry of the crystalline Form 2 of compound offormula (I), obtained as described in the previously cited patentapplication WO2013/174876 (on page 4, step c2) in ethanol is heated atreflux temperature until a solution is obtained and then cooled to atemperature between about 50° C. and about 65° C., preferably between55° C. and 60° C.

According to step g), the suspension of step f) is seeded with thecrystalline Form 4, then water is added (dripped within at least 15minutes) to obtain complete precipitation.

According to step h), the mixture of step g) is heated at refluxtemperature for a time period ranging from 30 to 90 minutes, preferablyfor about 60 minutes, and slowly cooled to room temperature. Afterwards,the mixture is stirred for at least 12 hours.

According to step i), the product obtained in step h) is filtered anddried under vacuum at about 40° C. to give the compound of formula (I)in crystalline Form 4.

It is understood that methods detailed herein may be carried out on aproduction or on a non-production scale.

The new procedure allows obtaining the crystalline Form 4 of compound offormula (I) with high purity and controlling the solid form. This formis suitable for development of an oral formulation.

Crystalline Form 4 as defined above may be administered by anyadministration route, for instance by oral, parenteral, topical, rectaland nasal route.

The compositions of the invention may be in a form suitable for oraluse. Examples of these forms are: tablets, hard or soft capsules,aqueous or oily suspensions, emulsions, dispersible powders or granules.The compositions of the invention may also be in a form suitable fortopical use. Examples of these forms are: creams, ointments, gels, oraqueous or oily solutions or suspensions. The compositions of theinvention may also be in a form suitable for administration byinhalation such as, for example, finely divided powder or a liquidaerosol. The compositions of the invention may also be in a formsuitable for administration by insufflation such as, for example, finelydivided powder. The compositions of the invention may also be in a formsuitable for parenteral administration (such as, for example, a sterileaqueous or oily solution for intravenous, subcutaneous, intramuscular)or as a suppository for rectal dosing.

The compositions of the invention may be obtained by conventionalprocedures using conventional pharmaceutical excipients, well known inthe art.

Thus, compositions intended for oral use may contain one or moreadditives such as, for example, colouring, sweetening, flavouring andpreservative agents.

For example, the solid oral forms may contain, together with the activecompound, acidulants, diluents, e.g., lactose, dextrose, saccharose,sucrose, mannitol, cellulose, corn starch or potato starch; lubricants,e.g., silica, talc, stearic acid, magnesium or calcium stearate, and/orpolyethylene glycols; glidants, e.g. colloidal silicon dioxide; bindingagents, e.g., starches, arabic gum, gelatine methylcellulose,carboxymethylcellulose or polyvinyl pyrrolidone; disintegrating agents,e.g., starch, alginic acid, alginates or sodium starch glycolate;effervescing mixtures; dyestuffs; sweeteners; wetting agents such aslecithin, polysorbates, laurylsulphates; and, in general, non-toxic andpharmacologically inactive substances used in pharmaceuticalformulations. These pharmaceutical preparations may be manufactured inknown manner, for example, by means of mixing, granulating, tabletting,sugar-coating, or film-coating processes.

The liquid dispersions for oral administration may be, e.g., syrups,emulsions and suspensions. As an example, the syrups may contain, ascarrier, saccharose or saccharose with glycerine and/or mannitol andsorbitol.

The suspensions and the emulsions may contain, as examples of carriers,natural gum, agar, sodium alginate, pectin, methylcellulose,carboxymethylcellulose, or polyvinyl alcohol.

The suspension or solutions for intramuscular injections may contain,together with the active compound, a pharmaceutically acceptablecarrier, e.g., sterile water, olive oil, ethyl oleate, glycols, e.g.,propylene glycol and, if desired, a suitable amount of lidocainehydrochloride.

The solutions for intravenous injections or infusions may contain, as acarrier, sterile water or preferably they may be in the form of sterile,aqueous, isotonic, saline solutions or they may contain propylene glycolas a carrier.

The suppositories may contain, together with the active compound, apharmaceutically acceptable carrier, e.g., cocoa butter, polyethyleneglycol, a polyoxyethylene sorbitan fatty acid ester surfactant orlecithin.

In another embodiment is provided crystalline Form 4 as defined abovefor use as a medicament.

In another embodiment is provided crystalline Form 4, as defined above,either alone or in association with other therapeutic agents orradiotherapy, for use in the treatment of a disease state treatable byinhibition of ALK, ROS1, TrkA, TrkB, TrkC, or a combination thereof.

In another embodiment are provided any of the methods of treatment of apatient having cancer, wherein said cancer contains at least one geneticalteration in at least one target gene selected from the groupconsisting of ALK, ROS1, NTRK1, NTRK2 and NTRK3.

A further object of the invention is to provide a method for treatingcancer in a mammal, including a human being, in need of ALK inhibitionand/or in a patient having at least one genetic alteration in at leastone target gene selected from the group consisting of ALK, ROS1, NTRK1,NTRK2 and NTRK3, such method comprising administering to said mammal atherapeutically effective amount of crystalline Form 4 as defined above.

A further object of the invention is to provide the use of thecrystalline Form 4 as defined above, either alone or in association withother therapeutically agents or radiotherapy, for the manufacture of amedicament for the treatment of a disease state treatable by ALKinhibition and/or in a patient having at least one genetic alteration inat least one target gene selected from the group consisting of ROS1,NTRK1, NTRK2 and NTRK3, selected from the group consisting of cancer andcell proliferative disorders.

As used in the present specification, the following words and phrasesare generally intended to have the meanings as set forth below, exceptto the extent that the context in which they are used indicatesotherwise.

In the paragraphs defining the x-ray powder diffraction peaks for thecrystalline Form 4 of compound of formula (I), the term “about”, usedfor instance in the expression “ . . . at a 2-theta value of about . . .” or “ . . . at about the 2-theta values . . . ”, indicates that theprecise positions of peaks (i.e. the recited 2-theta angle values)should not be considered as being absolute values because, as will beappreciated by those skilled in the art, the precise position of thepeaks may vary slightly between one machine and another, from one sampleto another, or as a result of slight variations in measurementconditions utilised. Generally, a measurement error of a diffractionangle in an x-ray powder diffractogram is about 2-theta=0.5 deg or less(or, more suitably, about 2-theta=0.2 deg or less) and such degree of ameasurement error should be taken into account when considering thex-ray powder diffraction patterns Therefore, where it is stated, forexample, that the crystalline Form 4 of compound of formula (I), have anx-ray powder diffraction pattern with at least one specific peak atabout 2-theta=17.9 degrees (or any one of the other mentioned angles),then this can be interpreted as being 2-theta=17.9±0.5 degrees, or2-theta=17.9±0.2 degrees. It should be understood that ±0.5 degrees canalso be expressed as “plus or minus 0.5 degrees 2θ”.

The use of the term “substantially the same as shown in” when referringto an x-ray powder diffraction pattern means that the 2-theta anglevalues of the pattern may vary slightly from one machine to another,from one sample to another, or as a result of slight variations inmeasurement conditions, so the peak positions shown in the figures orquoted in the tables are again not to be as absolute values. In thisregard, it is known in the art that an x-ray powder diffraction patternmay be obtained which has one or more measurement errors depending onmeasurement conditions (such as, for example, equipment and/or samplepreparation). In particular, it is generally known that intensities inan x-ray powder diffraction pattern may vary depending on measurementconditions and sample preparation. For example, persons skilled in theart of x-ray powder diffraction will realise that the relative intensityof peaks can be affected by, for example, grains above 30 microns insize and non-unitary aspect ratios, which may affect analysis ofsamples. The skilled person will also realise that the position ofreflections can be affected by the precise height at which the samplesits in the diffractometer and the zero calibration of thediffractometer. The surface planarity of the sample may also affect theresult.

Hence a person skilled in the art will appreciate that the diffractionpattern data presented herein are not to be considered as absolute (forfurther information see “Fundamentals of Powder Diffraction andStructural Characterization”, Pecharsky and Zavalij, Kluwer AcademicPublishers, 2003).

The term “disease state treatable” means that the treatment according tothe invention provides remission of the disease state or at least theconditions and quality of life of the mammal under treatment areimproved.

Examples of such disease states are in particular different cancers thatmay include specific types of cancer including carcinoma, squamous cellcarcinoma, hematopoietic tumors of myeloid or lymphoid lineage, tumorsof mesenchymal origin, tumors of the central and peripheral nervoussystem, melanoma, seminoma, teratocarcinoma, osteosarcoma, xerodermapigmentosum, keratocanthomas, thyroid follicular cancer and Kaposi'ssarcoma.

Examples of such disease states are specific types of cancer such as,but not restricted to, breast cancer, lung cancer, such as non-smallcell lung cancer, colorectal cancer, prostate cancer, ovarian cancer,endometrial cancer, gastric cancer, pancreatic cancer, papillary thyroidcancer, clear call renal cell carcinoma, uveal melanoma, multiplemyeloma, rhabdomyosarcoma, Ewing's sarcoma, Kaposi's sarcoma, andmedulloblastoma.

Other examples of such disease states are ALK+ Anaplastic Large CellLymphomas (ALCL) and possibly other indications in which the ALKactivity might play a role, like neuroblastoma, rhabdomyosarcoma,glioblastoma, inflammatory myofibroblastic tumor, and some kind ofmelanomas, breast carcinomas, Ewing's sarcomas, retinoblastomas andnon-small cell lung carcinomas (NSCLC).

Yet other examples of such disease states are cell proliferativedisorders such as, but not restricted to, benign prostate hyperplasia,familial adenomatosis polyposis, neurofibromatosis, psoriasis, vascularsmooth cell proliferation associated with atherosclerosis, pulmonaryfibrosis, arthritis, glomerulonephritis and post-surgical stenosis andrestenosis.

In some embodiments, the crystalline form disclosed herein is formulatedfor oral administration using pharmaceutically acceptable carriers wellknown in the art.

In some embodiments, the therapeutically effective dose of thecrystalline Form 4 of compound of formula (I), as defined above, or apharmaceutically acceptable salt, may vary according to the disease,severity of the disorder and the conditions of the patient to betreated. Therefore the physician, as always, must set the optimal dosefor each patient. Anyway, the effective dosage range may be from about10 mg to about 1 g per dose (calculated as a free base), from 1 to 3times daily.

In some embodiments, the therapeutically effective dose of thecrystalline Form 4 of compound of formula (I) is between about 100 mgand about 2000 mg, or between about 100 mg and about 1750 mg, or betweenabout 100 mg and about 1500 mg, or between about 100 mg and about 1250mg, or between about 150 mg and 1250 mg, or between about 175 mg andabout 1250 mg, or between about 200 mg and about 1250 mg, or between 250mg and about 1250 mg, or between about 300 mg and about 1250 mg, orbetween about 350 mg and about 1250 mg, or between about 400 mg andabout 1250 mg, or between about 400 mg and about 1000 mg, or betweenabout 450 mg and about 950 mg, or between about 450 mg and about 900 mg,or between about 450 mg and about 850 mg, or between about 500 mg andabout 850 mg, or between about 500 mg and about 800 mg, or between about525 mg and about 800 mg, or between about 525 mg and about 775 mg, orbetween about 550 mg and about 800 mg, or between about 575 mg and about800 mg, or between about 575 mg and about 775 mg, or between about 600mg and 800 mg, or between about 600 mg and 775 mg, or between about 600mg and 750 mg, or between about 600 mg and about 725 mg, or betweenabout 500 mg and about 725 mg, or about between about 500 mg and about700 mg.

n some embodiments, the therapeutically effective dose of thecrystalline Form 4 of compound of formula (I) is about 250 mg, about 275mg, about 300 mg, about 325 mg, about 350 mg, about 375 mg, about 400mg, about 425 mg, about 450 mg, about 475 mg, about 500 mg, about 525mg, about 550 mg, about 600 mg, about 625 mg, about 650 mg, about 675mg, about 700 mg, about 725 mg, about 750 mg, about 775 mg, about 800mg, about 825 mg, about 850 mg, about 875 mg, about 900 mg, about 925mg, about 950 mg, about 975 mg, or about 1000 mg. In some embodiments,the therapeutically effective dose of the crystalline Form 4 of compoundof formula (I) is about 600 mg, taken orally once per day.

EXPERIMENTAL SECTION

The following examples are included to illustrate embodiments of thedisclosure, and are not intended to limit the scope of the disclosure.

The crystalline form described herein may be characterized by variousmethods known in the art, such as x-ray powder diffraction pattern(XRPD) and differential scanning calorimetry (DSC), including, forexample, the methods described in Examples 3 and 4.

In one embodiment of the present invention, the crystalline Form 4 ischaracterized by an x-ray diffraction diagram that is substantially thesame as the diagram shown in FIG. 1, with significant peak intensitiesat about the 2-theta values (deg) described in Table 1. In samples beingfree of any additional materials (other crystalline forms, excipients),it should be possible to observe the diffraction peaks at about the2-theta values (deg) described in Table 2.

TABLE 1 Description of the solid state properties and Figures/Tablereferences of crystalline Forms 4 of the compound of formula (I).Compound (I) XRPD DSC XRPD peaks (2-theta, deg) Polymorph FIG. 1 FIG. 28.6, 15.6, 17.9, 19.0, Form 4 Table 2 19.2, 19.6, 19.7, 20.7, 21.3,22.1, 22.7, 24.3, 24.6

In the following Examples, the temperatures are measured in degreesCelsius (° C.) and, unless otherwise indicated, the reactions orexperiments take place at room temperature (RT).

Example 1: Preparation of Crystalline Form 4 of Compound of Formula (I)

Steps a) and b) are Described in the Application WO2013174876 on Page 4,Lines 15-28.

Step c)

2.88 Kg of the dried amorphousN-[5-(3,5-difluorobenzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl-2-(tetrahydro-pyran-4-ylamino)-benzamide(compound of formula (I)), obtained by the procedure described inWO20131174876 (steps a and b on pages 3-4), are suspended in about 10volumes of ethanol (suspension A).

Step d)

20 mL of the suspension A obtained in Step c) are heated to about 60° C.to get a solution, then the solution is cooled to room temperature.

Step e)

20 mL of water are added to the suspension obtained from Step d), andthe precipitate is filtered. The product is dried under vacuum to giveN-[5-(3,5-difluorobenzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamideas crystalline Form 4 (DSC analysis is reported in FIG. 2).

Example 2: Preparation of the Crystalline Form 4 by Seeding

Step f)

110 mL of ethanol are added to 11.3 g of crystalline Form 2 of2N-[5-(3,5-difluorobenzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide,obtained as described in WO2013/174876 (step c2, page 4 and 11-12), andthe mixture is heated at reflux until a solution is obtained. Themixture is then cooled to about 58° C.

Step g)

20 mg of seeds of the crystalline Form 4 prepared as described in theExample 1 are added to the mixture of step f); 220 mL of water are thendripped into the mixture within about 15 min obtaining completeprecipitation.

Step h)

The mixture thus obtained is heated again to reflux for about 60 min andslowly cooled to room temperature; then it is stirred at roomtemperature for 12 hours.

Step i)

The precipitate is filtered and dried under vacuum at about 40° C.yielding 9.5 g of crystalline Form 4 ofN-[5-(3,5-difluorobenzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamide.

Example 3: Analytical Results by Means of X-Ray Powder Diffraction(XRPD)

The crystalline Form 4 of compound (I) was characterized by x-ray powerdiffraction (XRPD) performed using a Thermo/ARL XTRA apparatus,irradiating powder samples with a CuKα source (45 kV, 40 mA, 1.8 kW-Kα1radiation, wavelength λ=1.54060 Angstrom) between 2° and 40° 2-theta atroom temperature.

The scan rate was of 1.20°/min (0.020° step with count time of 1 secondsper step).

In the x-ray diffractograms, the angles of diffraction 2-theta areplotted on the horizontal axis (x-axis) and the line intensity on thevertical (y-axis).

As reported in the preceding paragraphs, the crystalline Form 4 ofcompound of formula (I) provides x-ray powder diffraction patternssubstantially the same as the x-ray powder diffraction pattern shown inFIG. 1 and has substantially the most prominent peaks at the 2-thetaangle values shown in Tables 1 and 2.

Therefore, it shall be understood that the crystalline Form 4 ofcompound of formula (I) described in the present invention is notlimited to the crystal that provide x-ray powder diffraction patternidentical to the x-ray powder diffraction pattern shown in FIG. 1 andany sample or batch of crystalline Form 4 of compound of formula (I)providing x-ray powder diffraction pattern substantially the same asthat shown in FIG. 1 here below falls within the scope of the presentinvention. A person skilled in the art of x-ray powder diffraction isable to judge the substantial identity of x-ray powder diffractionpatterns.

The x-ray diffraction peak positions of crystalline Form 4 is reportedin the following Table 2.

TABLE 2 Crystalline Form 4 of the compound of formula (I) Position(Deg.) Intensity (CPS) Relative Intensity (%) 8.6 202.8 5.4 10.3 102.02.7 11.0 206.5 5.5 11.9 26.6 0.7 14.3 146.3 3.9 14.6 68.4 1.8 15.1 78.92.1 15.3 233.9 6.3 15.6 992.9 26.6 16.1 88.8 2.4 17.1 253.2 6.8 17.93739.3 100.0 19.0 478.0 12.8 19.2 598.8 16.0 19.6 795.1 21.3 19.7 711.319.0 20.1 260.3 7.0 20.7 723.7 19.4 21.3 1304.4 34.9 22.1 459.8 12.322.7 502.1 13.4 24.3 525.6 14.1 24.6 420.4 11.2 25.3 54.0 1.4 25.5 122.33.3 25.9 255.9 6.8 26.7 316.6 8.5 26.9 41.5 1.1 27.3 107.4 2.9 27.7111.2 3.0 28.1 107.3 2.9 28.6 116.8 3.1 29.0 55.3 1.5 29.5 22.7 0.6 29.956.0 1.5 30.5 65.7 1.8 31.0 153.5 4.1 31.6 42.4 1.1 32.2 176.8 4.7 33.3150.8 4.0 34.0 73.5 2.0 35.4 56.1 1.5 36.4 70.4 1.9 36.8 39.4 1.1 39.042.0 1.1

Example 4: Analytical Results by Means of Differential ScanningCalorimetry (DSC)

DSC analyses were carried out with a Mettler Toledo Star systemapparatus. Aluminum DSC pans were loaded with 2-4 mg of sample. Thetemperature range of the analyses was between 25° C. and a maximum valueof 300° C. The samples were analyzed under nitrogen static condition ata heating rate of 10° C./min.

FIG. 2 reports DSC thermograms of the crystalline Form 4.

The observed melting endotherm for crystalline Form 4 is atapproximately in the range 200° C.-205° C. (peak temperature) with DeltaH in the range 70-82 J/g. It will be understood that the onset and/orpeak temperature values of the DSC may vary slightly from one apparatusto another, one method to another or from one sample to another, and sothe values quoted are not to be considered as absolute. In fact,observed temperatures will depend on the rate of temperature change aswell as sample preparation technique and the particular instrumentemployed. It will be estimated and taken into account that thetemperature values obtained applying such different conditions may varyby plus or minus about 4° C.

1.-18. (canceled)
 19. A method of treating cancer in a mammalcomprising: administering to said mammal a therapeutically effectiveamount of crystalline Form 4 ofN-[5-(3,5-difluoro-benzyl)-1H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamideof the following formula (I)

wherein said crystalline form is characterized by an X-ray powderdiffraction pattern comprising peaks at 2-theta values of about 8.6,15.6, 17.9, and 21.3±0.2 degrees.
 20. The method of claim 19, whereinthe mammal is a human being.
 21. The method of claim 19, wherein saidX-ray powder diffraction pattern further comprises the peaks at 2-thetavalues of about 19.7, 22.1, and 24.3±0.2 degrees.
 22. The method ofclaim 19, wherein said crystalline form is further characterized byexhibiting a peak in a differential scanning calorimetry scan of fromabout 200° C. to about 205° C.
 23. The method of claim 19, wherein saidcrystalline form is further characterized by a Delta H in the range70-82 J/g.
 24. The method of claim 19, wherein said X-ray powderdiffraction pattern further comprises the peaks at 2-theta values ofabout 19.6 and 22.7±0.2 degrees.
 25. The method of claim 24, whereinsaid X-ray powder diffraction pattern further comprises the peaks at areflection angle 2-theta of about 10.3, 11.0, 11.9, 14.3, 14.6, 15.1,15.3, 16.1, 17.1, 19.0, 19.2, 20.1, 20.7, 24.6, 25.3, 25.5, 25.9, 26.7,26.9, 27.3, 27.7, 28.1, 28.6, 29.0, 29.5, 29.9, 30.5, 31.0, 31.6, 32.2,33.3, 34.0, 35.4, 36.4, 36.8, and 39.0±0.2 degrees.
 26. The method ofclaim 19, wherein the cancer is selected from the group consisting ofbreast cancer, lung cancer, colorectal cancer, prostate cancer, ovariancancer, endometrial cancer, gastric cancer, clear cell renal cellcarcinoma, invasive ductal carcinoma (breast), uveal melanoma, multiplemyeloma, rhabdomyosarcoma, Ewing's sarcoma, Kaposi's sarcoma, pancreaticcancer, medulloblastoma, anaplastic large cell lymphomas (ALCL),neuroblastoma, rhabdomyosarcoma, glioblastoma, inflammatorymyofibroblastic tumor, melanoma, retinoblastoma, non-small cell lungcancer (NSCLC), salivary gland cancer, and mammary analog secretorycarcinoma (MASC).
 27. The method of claim 19, wherein the cancer isselected from the group consisting of breast cancer, lung cancer,colorectal cancer, prostate cancer, gastric cancer, medulloblastoma,neuroblastoma, glioblastoma, melanoma, non-small cell lung cancer(NSCLC), salivary gland cancer, and mammary analog secretory carcinoma(MASC).
 28. The method of claim 19, wherein the cancer is selected fromthe group consisting of breast cancer, lung cancer, colorectal cancer,medulloblastoma, neuroblastoma, glioblastoma, melanoma, non-small celllung cancer (NSCLC), salivary gland cancer, and mammary analog secretorycarcinoma (MASC).
 29. The method of claim 19, wherein the cancer isselected from the group consisting of non-small cell lung cancer,papillary thyroid cancer, neuroblastoma, pancreatic cancer andcolorectal cancer.
 30. A method of treating cancer in a mammal having atleast one genetic alteration in at least one target gene selected fromthe group consisting of ROS1, NTRK1, NTRK2 and NTRK3, comprising:administering to said mammal a therapeutically effective amount ofcrystalline Form 4 ofN-[5-(3,5-difluoro-benzyl)-H-indazol-3-yl]-4-(4-methyl-piperazin-1-yl)-2-(tetrahydro-pyran-4-ylamino)-benzamideof the following formula (I)

wherein said crystalline form is characterized by an X-ray powderdiffraction pattern comprising peaks at 2-theta values of about 8.6,15.6, 17.9, and 21.3±0.2 degrees.
 31. The method of claim 30, whereinthe mammal is a human being.
 32. The method of claim 30, wherein saidX-ray powder diffraction pattern further comprises the peaks at 2-thetavalues of about 19.7, 22.1, and 24.3±0.2 degrees.
 33. The method ofclaim 30, wherein said crystalline form is further characterized byexhibiting a peak in a differential scanning calorimetry scan of fromabout 200° C. to about 205° C.
 34. The method of claim 30, wherein saidcrystalline form is further characterized by a Delta H in the range70-82 J/g.
 35. The method of claim 30, wherein said X-ray powderdiffraction pattern further comprises the peaks at 2-theta values ofabout 19.6 and 22.7±0.2 degrees.
 36. The method of claim 35, whereinsaid X-ray powder diffraction pattern further comprises the peaks at areflection angle 2-theta of about 10.3, 11.0, 11.9, 14.3, 14.6, 15.1,15.3, 16.1, 17.1, 19.0, 19.2, 20.1, 20.7, 24.6, 25.3, 25.5, 25.9, 26.7,26.9, 27.3, 27.7, 28.1, 28.6, 29.0, 29.5, 29.9, 30.5, 31.0, 31.6, 32.2,33.3, 34.0, 35.4, 36.4, 36.8, and 39.0±0.2 degrees.
 37. The method ofclaim 30, wherein the cancer is selected from the group consisting ofbreast cancer, lung cancer, colorectal cancer, prostate cancer, ovariancancer, endometrial cancer, gastric cancer, clear cell renal cellcarcinoma, invasive ductal carcinoma (breast), uveal melanoma, multiplemyeloma, rhabdomyosarcoma, Ewing's sarcoma, Kaposi's sarcoma, pancreaticcancer, medulloblastoma, anaplastic large cell lymphomas (ALCL),neuroblastoma, rhabdomyosarcoma, glioblastoma, inflammatorymyofibroblastic tumor, melanoma, retinoblastoma, non-small cell lungcancer (NSCLC), salivary gland cancer, and mammary analog secretorycarcinoma (MASC).
 38. The method of claim 30, wherein the cancer isselected from the group consisting of breast cancer, lung cancer,colorectal cancer, prostate cancer, gastric cancer, medulloblastoma,neuroblastoma, glioblastoma, melanoma, non-small cell lung cancer(NSCLC), salivary gland cancer, and mammary analog secretory carcinoma(MASC).
 39. The method of claim 30, wherein the cancer is selected fromthe group consisting of breast cancer, lung cancer, colorectal cancer,medulloblastoma, neuroblastoma, glioblastoma, melanoma, non-small celllung cancer (NSCLC), salivary gland cancer, and mammary analog secretorycarcinoma (MASC).
 40. The method of claim 30, wherein the cancer isselected from the group consisting of non-small cell lung cancer,papillary thyroid cancer, neuroblastoma, pancreatic cancer andcolorectal cancer.